Can the Visio desktop app be used in a virtualized environment? Yes. This article explains what is allowed:
If you have the Visio Plan 2 subscription
You may install and use licensed copies of Visio on:
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Dedicated hardware Physical servers maintained and used only by your organization, as long as the server hardware isn't provided by a service from Alibaba, Amazon, or Google. Multi-tenancy isn't allowed.
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Azure Either dedicated hardware or on multi-tenant architecture.
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Certain third-party multi-tenant hosts Microsoft has a program that qualifies third-party hosts for this kind of arrangement. Click here to download a list a qualified third-party hosts. For details of the program, see Qualified Multi-tenant Hoster Program.
If you have a perpetual version of Visio
You may install and use licensed copies of Visio on:
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Dedicated hardware Physical servers maintained and used only by your organization, as long as the server hardware isn't provided by a service from Alibaba, Amazon, or Google.
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A multi-tenant host The host must have a Services Provider Licensing Agreement with Microsoft. An SPLA provides the license rights to host specific Microsoft products.