You cannot start a computer from a USB flash drive that is formatted to use the FAT32 file system


You format a USB flash drive to use the FAT32 file system. When you try to start the computer from this USB flash drive, the startup process stops responding, and the screen is black.


This issue occurs because the USB flash drive is listed as removable media. Therefore, the Windows operating system does not create a master boot record (MBR) on the USB flash drive when you format the flash drive to use the FAT32 file system. The USB flash drive is treated as a super floppy disk. The FAT32 startup code does not support starting a computer from a super floppy disk without an MBR.

The BIOS tries to transfer the control of the startup from the USB flash drive to the FAT32 startup code, even though the FAT32 startup code does not support this scenario.


To work around this issue, use the Diskpart command prompt utility to create and format the boot partition on the USB flash drive.

For more information about how to use Diskpart, click the following article number to view the article in the Microsoft Knowledge Base:

300415 A Description of the Diskpart Command-Line Utility

More Information

How to differentiate between the MBR and the boot sector

Currently, the Windows operating system uses signatures at offset 3 in the boot sector to determine whether the sector is a boot sector. These signatures do not appear in the MBR. The signatures are as follows:
  • FAT16: MSDOS5.0
  • FAT32: MSDOS5.0

How to determine whether the boot sector is FAT32, FAT16, or NTFS

To determine whether the USB flash drive was formatted by using the FAT32, FAT16, or NTFS file system, you must check two strings in the boot sector. If the strings contain FAT32,FAT16 or NTFS, the boot sector was formatted in that particular file system format.